FastQCFastQC Report
Mon 27 Feb 2023
SRR3128009.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128009.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2800272
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT239660.855845432158019No Hit
GCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTG52940.18905306341669667No Hit
CCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTG43100.15391361981978893No Hit
CTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC40220.143628904620694Illumina Single End Adapter 2 (95% over 21bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT36120.1289874697886491No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT73200.080.6327741
ATAGGGC16200.074.555383
GGTAAGG16900.074.302511
AGGGATG41500.072.475765
TAGGGTA9650.072.076774
TAGGGCA12950.071.854894
CGTAGGG7500.071.433922
GTAGGGT10850.070.1691363
TATAGGG21300.070.163052
AGTAGGG44700.070.126022
ATAGGGA23700.069.998273
AAGGGTA20000.069.554084
TAAGGGA24200.069.523013
ACTAGGG11450.069.365272
AGGGTAC13800.069.13185
AGTACGG3950.069.057341
TACGGGT3200.069.0253753
AAGGGAT49950.068.6828464
AGGGAAT30150.068.27285
GTACGGG6300.067.883112
Produced by FastQC (version 0.11.5)