FastQCFastQC Report
Mon 27 Feb 2023
SRR3128008.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128008.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3503114
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT350521.0005954702016548No Hit
GCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTG53760.15346346136608743No Hit
CCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTG46380.1323964906651625No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT45350.1294562494968762No Hit
CTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC43120.12309048463738262Illumina Single End Adapter 2 (95% over 21bp)
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36130.10313680913610006No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT103450.081.4099961
TAGGGTA12750.072.616224
ATAGGGC19150.072.398713
GGTAAGG22100.072.170981
TAGGGCA18100.070.88624
AGGGATG51400.070.772035
CGTAGGG9900.070.259352
AGGGTAC16600.070.21465
AGTAGGG57400.070.0873262
TAAGGGA31700.069.977813
TAGAGGG59700.069.3552252
ATAGAGG23300.069.0598141
AGGGAAT37300.067.78875
AAGGGTA23400.067.484044
GTAGGGT14650.066.7272643
ACGGGTA4650.066.7065664
AGTACGG5450.066.473631
TATAGGG25250.066.262262
ACTAGGG15500.066.100142
TAGTAGG18300.065.817911
Produced by FastQC (version 0.11.5)