FastQCFastQC Report
Mon 27 Feb 2023
SRR3128007.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128007.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2900175
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT483561.6673476600549968No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT37550.1294749454774281No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT146850.087.298291
ATAGGGC16900.074.527023
TAGGGCA14450.074.153674
ACGGGTA3950.073.7666854
ATAGGGA26900.070.931553
AGGGTAC14650.069.933395
GAATAGG16650.068.968091
TAGGGTA8500.068.559624
AGTAAGG15750.068.128191
AGTAGGG50450.068.121882
TACGGGT3250.067.9641953
TAGGGAT19300.067.937974
TAGTAGG14850.067.503411
AGGGAAT33150.067.3404165
AGGGATG41850.066.367845
AGATAGG17450.066.345621
ATAGAGG20900.066.2023851
GTAGGGA22050.065.859073
TAGAGGG55350.065.65872
AATAGGG43250.065.548332
Produced by FastQC (version 0.11.5)