FastQCFastQC Report
Mon 27 Feb 2023
SRR3128006.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128006.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2708440
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT439761.6236652833365333No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT33040.12198904166235915No Hit
CCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTG27300.10079603018711877No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT135750.086.760371
ATAGGGC16200.073.68733
TAGGGCA13250.071.647574
ATAGGGA24700.070.971813
GAATAGG15100.070.709181
ATAGAGG18250.069.844681
AGGGATG37450.068.769325
AGTAGGG48200.068.6435242
TATAGGG20000.066.971312
ATAAGGG38150.066.400022
GTAGGGA21800.066.184443
TAGAGGG51250.066.117342
TAGCGGG13950.066.032212
AGATAGG16650.065.8216251
GGTAAGG16550.065.082521
ATGTAGG7950.065.080741
ACGGGAT8150.064.584214
AATAGGG38800.064.560882
GAGGGAT30250.064.47464
GATAGGG40550.064.208562
Produced by FastQC (version 0.11.5)