FastQCFastQC Report
Mon 27 Feb 2023
SRR3128005.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128005.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3382986
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT628771.8586243040911192No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT41150.12163810314319953No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT189100.087.7391361
TAGGGCA17950.074.884224
ATAGGGC22500.073.109573
ATAGGGA34250.070.944493
GAATAGG20350.069.599251
ACGGGTA4150.069.082884
TACGGGT3000.068.931883
ACGGGAT9550.068.899084
AAGGGTA26750.068.521924
ATAACGG3250.068.048261
AGGGATG52600.067.818965
AGTAGGG60350.067.597582
CGTAGGG9850.067.277772
TAGAGGG63200.067.152072
ATAAGGG50200.066.47272
ATAGAGG24000.066.072571
AGATAGG22600.066.0014341
GATAGGG53500.065.710762
TAGGGTA11750.064.798644
GGGCGAT16450.063.9995966
Produced by FastQC (version 0.11.5)