FastQCFastQC Report
Mon 27 Feb 2023
SRR3128004.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128004.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2015287
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT758823.765319778274757No Hit
CGTTCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTT62090.3080950752920056No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCT60240.29891524135272046No Hit
CGCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCT50970.25291683020830286No Hit
CCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTG31740.1574961779637342No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTC27180.13486912782149638No Hit
CGTCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTC24580.12196773958250116No Hit
CTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGC22590.1120932155072702No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT21610.10723038455564889No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT219150.090.17231
ATAGGGC14150.075.728373
AGGGATG33350.072.716685
TAAGGGA16900.071.7485663
GGTAAGG12750.070.483681
TAGGGAT15550.069.5148544
ATAGGGA19900.069.198193
ATAGCGG3300.068.437261
AGGGCGA9550.068.40565
TAGGGCA9650.068.183764
GAGGGAT24300.067.499314
AGTAGGG31350.067.481472
AGAGGGC28950.067.20973
TAGAGGG32350.066.5580752
AAGGGAT33300.066.051374
GTTTTTT302100.066.0285642
GTAGGGA16150.065.477223
AGGGTAC7700.065.309035
TAGACGG3250.065.147011
GAGGGTA10700.064.567474