FastQCFastQC Report
Mon 27 Feb 2023
SRR3128003.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128003.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1891910
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT696093.679297641008293No Hit
CGTTCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTT66080.34927665692342663No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCT64740.34219386757298176No Hit
CGCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCT54750.2893900872663076No Hit
CCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTG33410.17659402402862714No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTC29250.15460566306008214No Hit
CGTCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTC26410.13959437816809467No Hit
CTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGC23650.12500594637165616No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT18990.10037475355593026No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT200400.090.024941
AGGGATG32400.073.8320165
TAGGGCA8950.071.939854
CGAGGGA8850.071.161483
ACGGGAT6000.070.4958044
TAGGGTA6350.070.3107764
GAGGGAT22950.070.2398154
ATAGGGC11750.069.997683
ATAGGGA16400.068.491633
GAATAGG11300.067.8408361
ATAACGG1600.067.606711
TAAGGGA15300.066.9650963
CGTTTCT9850.066.845741
AGTAGGG27150.066.8191452
AAGAGGG60200.065.89152
AGAGGGC27200.065.6595843
GGATGGC27200.065.485077
GTAGGGT7250.065.473693
TAGAGGG32400.065.420672
AAGGGAT29200.065.345424