FastQCFastQC Report
Mon 27 Feb 2023
SRR3128001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3040626
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT312471.027650227288723No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC57370.188678252438807No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT115400.084.341011
AGGGATG69400.076.318475
ATAGCGG6300.075.452731
CGGGATG15100.073.140195
CGTAGGG8200.072.2449652
TAGACGG3950.071.4904861
GGATGGC57550.071.454227
GAGGGAT51850.071.151834
GTAGGGA27950.070.2845153
AGAGGGC41800.070.269843
TAGCGGG16000.069.937142
TAGGGTC10050.069.676354
TAGGGCA19250.069.335044
TAGAGGG54050.068.8068542
ATAGGGC20750.068.626163
AAGAGGG107150.067.968892
TAAGGGA33000.067.931253
AGTAGGG49900.067.651092
ATGCGGG13450.067.466152
CGAAGGG27800.067.3114852
Produced by FastQC (version 0.11.5)