FastQCFastQC Report
Mon 27 Feb 2023
SRR3128000.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128000.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2790852
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT285751.0238808793873697No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC50830.18213076150222227No Hit
GCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTG28180.10097274954028376TruSeq Adapter, Index 16 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT100650.085.327241
AGGGATG60550.077.538495
ATAGCGG5850.073.9660951
TAGGGCA16100.071.8071064
ATAGGGC18600.071.2515643
AGTAGGG45450.070.829612
GGATGGC48350.070.276137
CGGGATG12900.069.948365
CGTAGGG7350.069.694312
ACGGGAT11750.069.593644
TAGAGGG49650.069.381382
GAGGGAT45350.069.1204764
GTAGGGA27900.068.893353
AGTAAGG18650.068.846921
AGAGGGC41050.068.461483
TAGGGAT26000.068.143784
TAAGGGA28700.068.119223
AAGGGAT60450.067.947484
TAGCGGG17000.067.72912
GTAAGGG42000.067.69622
Produced by FastQC (version 0.11.5)