FastQCFastQC Report
Mon 27 Feb 2023
SRR3127999.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127999.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3504461
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT398561.1372932956023767No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC66000.18833138676675243No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT137650.085.606211
AGGGATG81900.073.969435
AGTAGGG59000.072.490122
TAGGGCA21700.072.339614
ATAGCGG7700.070.86651
GAGGGAT60600.070.73134
GGATGGC62300.070.309037
ATAGGGA43150.070.035723
CGGGATG16900.069.8023765
TAGCGGG20250.069.396192
ATAGGGC24700.068.310423
TAGAGGG60550.068.3058552
GTAGGGA34350.068.0015953
AGAGGGC46450.067.994383
CGTAGGG9250.067.5770652
TACGACC13850.067.528416
AAGGGCA62000.067.239014
CGAAGGG32200.066.849632
TAGGGAT35100.066.816374
AGGGATC32050.066.428345
Produced by FastQC (version 0.11.5)