FastQCFastQC Report
Mon 27 Feb 2023
SRR3127996.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127996.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1287290
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT157221.2213254200685162No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC33990.2640430672187308No Hit
CCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTG27190.211218917260291No Hit
CTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGC26530.2060918674113836No Hit
GCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTG25510.19816824491761764No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13810.10727963395971382No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT46000.083.960491
AGGGATG30750.081.623355
ATAACGG1600.079.384081
TAGGGTA4800.079.3100744
AGTACGG1550.078.909871
ACGGGTA2050.077.948844
CGTAGGG3150.077.584932
AGGGTAC6400.077.113015
GGATGGC24250.076.7541357
AGGGAAT21900.075.761565
GAGGGAT21150.075.5532464
TAAGGGA14700.075.133763
GTACGGG2750.073.488662
CGAGGGA7350.072.895733
AGTAGGG22050.072.682592
GTAGGGA10250.072.446563
AAGGGCA18900.072.11424
GGGATGA21250.071.664556
GAGGGTA8750.071.437814
AGGGTAT8300.071.352765
Produced by FastQC (version 0.11.5)