FastQCFastQC Report
Mon 27 Feb 2023
SRR3127995.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127995.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2798786
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT319081.1400657284979987No Hit
GCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTG28450.10165121592004533No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT101350.084.6174241
ATAGGGC18500.077.225633
TAGTAGG10650.072.039921
ACGGGTA3300.071.205864
GAATAGG18350.070.795911
TAGGGCA15500.070.645394
AGGGATG39300.069.955675
AGTAGGG44350.069.3437962
ATAACGG2550.068.296231
GGTAAGG16600.068.05161
GTACGGG7900.067.858032
TAAGGGA24500.067.71243
AAGGGAT46050.067.151534
ATAAGGG38400.066.617942
AGTAAGG15850.066.5202561
TAGGGTC8800.066.221444
AGAGGGC45800.066.184133
TAGAGGG52200.066.1224752
AAGAGGG95450.065.868692
GTAGGGA24500.065.7942053
Produced by FastQC (version 0.11.5)