FastQCFastQC Report
Mon 27 Feb 2023
SRR3127994.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127994.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2628311
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT289321.1007829743131616No Hit
GCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTG31080.11825084626590993No Hit
CCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTG27390.10421141181542062No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT85550.084.1081541
CGTAGGG7400.074.943842
AGGGATG38550.069.856635
ATAGGGC15950.069.8336943
ATAGCGG5350.069.444841
GAATAGG17450.069.263491
AGTAAGG16550.068.7676241
AATAGGG38850.068.7136462
TAGAGGG48100.068.592672
AGAGGGC38500.068.238343
TAGGGCA13100.067.805964
AGGGCAT22550.067.735175
GTACGGG5850.067.4852752
GGTAAGG15900.067.437951
GAGGGAT33200.066.816134
AGTAGGG41250.066.6526642
TAGTAGG8900.066.580521
AAGAGGG87500.065.798152
GTAGGGC12500.065.79693
ATAAGGG34800.065.230922
Produced by FastQC (version 0.11.5)