FastQCFastQC Report
Mon 27 Feb 2023
SRR3127992.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127992.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1114091
Sequences flagged as poor quality0
Sequence length100
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128181.1505343818413398No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC77670.697160285829434No Hit
GCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTG56000.5026519377680998TruSeq Adapter, Index 22 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTG50290.45139939197067386TruSeq Adapter, Index 22 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGC48610.43631983383763084TruSeq Adapter, Index 20 (95% over 21bp)
AAAGCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTT22400.20106077510723988No Hit
TAGACCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCT21900.19657281137716756No Hit
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC21120.18957158795825474No Hit
TCCGCTACGACCAACTCATACACCTGTCTCTTATACACATCTGACGCTCT20210.18140349396952313No Hit
AGCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCT17490.15698897127792974No Hit
CGCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCT16550.14855159946539376No Hit
GGCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCT15390.138139523611626No Hit
ACTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTG14710.13203589293872764No Hit
ACCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCT12810.11498163076445282No Hit
AGAGCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTT12690.11390451946923547No Hit
GAACCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTT12280.11022438921057616No Hit
TCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTG12170.10923703718996025TruSeq Adapter, Index 22 (95% over 21bp)
GAATCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTT11350.10187677667264164No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCGG1750.091.4484251
CGTTTTT36550.086.153731
AGGGATG36150.083.983965
GCGGGAT6550.083.231714
ATGGCAC31100.082.2073449
GGATGGC32900.082.137997
ACGGGTA1500.081.4618454
CGCTACG15500.081.2597053
GGATGAC20200.081.1981667
CGAAGGG7700.080.610082
GATGGCA31850.080.4191068
TCCGCTA15900.080.2245941
GCTACGA15700.080.224554
TAAGGGA13400.079.263973
AGGGCAT10100.079.104235
CTACGAC16050.079.060745
TAGGGCA5600.078.888184
AAGGGCA14800.078.752094
GAATGCC14650.078.596038
AAGGGAT33250.078.305144
Produced by FastQC (version 0.11.5)