FastQCFastQC Report
Mon 27 Feb 2023
SRR3127991.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127991.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1039197
Sequences flagged as poor quality0
Sequence length100
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116881.1247145632637507No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC70090.6744630710057862No Hit
GCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTG60920.5862218616874375TruSeq Adapter, Index 22 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTG54200.5215565479884949TruSeq Adapter, Index 22 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGC54060.5202093539531003TruSeq Adapter, Index 20 (95% over 21bp)
AAAGCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTT25840.24865352767569573No Hit
TAGACCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCT24560.23633632506637337No Hit
AGCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCT19500.1876448835013958No Hit
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC19280.18552786430291848No Hit
TCCGCTACGACCAACTCATACACCTGTCTCTTATACACATCTGACGCTCT18260.17571259347361473No Hit
CGCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCT17530.16868793886048555No Hit
GGCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCT16400.15781415843194313No Hit
ACTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTG16020.15415748890730052No Hit
ACCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCT14210.1367401945925556No Hit
AGAGCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTT13550.13038913699712373No Hit
GAACCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTT13330.12827211779864645No Hit
TCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTG13070.12577018601862786TruSeq Adapter, Index 22 (95% over 21bp)
GAATCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTT12630.12153614762167328No Hit
GGAACTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTT12270.11807193438780135No Hit
AATGCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTT11310.10883403243080955No Hit
GAAGCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTT10610.10209806225383639No Hit
AGACCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTT10570.10171314967229504No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGCG1300.086.769094
AGGGATG33150.086.0601965
TACGGGT550.085.4544143
CGTTTTT33300.085.2737351
GGATGGC30700.085.1162957
CGAGGGA5700.084.105133
CGTAGGG2800.083.928442
GACCGAT452.7284841E-1183.551418
CGCTACG13050.083.1952743
ACGGGTA1250.082.719874
GATGGCA29650.082.582748
TCGATGG401.033186E-982.273631
ATGGCAC29650.082.1072169
GCGGGAT7700.081.792084
CTACGAC13300.081.6314545
TAGCGGG4100.081.390122
AGGGTAT7300.081.123175
GAGGGTA6650.080.5713044
GCTACGA13500.080.42214
GATGCGG1700.080.199491
Produced by FastQC (version 0.11.5)