FastQCFastQC Report
Mon 27 Feb 2023
SRR3127990.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127990.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1249837
Sequences flagged as poor quality0
Sequence length100
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170571.3647379618302227No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC90750.7260946827466302No Hit
GCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTG61290.49038394606656704TruSeq Adapter, Index 22 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGC58230.465900753458251TruSeq Adapter, Index 20 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTG58190.4655807117248089TruSeq Adapter, Index 22 (95% over 21bp)
AAAGCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTT26750.2140279092393648No Hit
TAGACCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCT26550.2124277005721546No Hit
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC23670.18938469576432768No Hit
TCCGCTACGACCAACTCATACACCTGTCTCTTATACACATCTGACGCTCT20160.1613010336547886No Hit
AGCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCT19950.15962081455421787No Hit
CGCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCT17310.13849806014704316No Hit
ACTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTG16570.1325772880783654No Hit
GGCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCT15430.12345609867526726No Hit
ACCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCT14900.11921554570716023No Hit
TCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTG14520.11617514923946083TruSeq Adapter, Index 22 (95% over 21bp)
AGAGCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTT13850.11081445020430664No Hit
GAACCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTT13550.10841413720349133No Hit
GAATCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTT13400.10721398070308369No Hit
GCGCAGTGATTATAGGCTTTCGCTCTAAGATTAAAAATGCCCTAGCCCAC12770.10217332340137154No Hit
AATGCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTT12710.10169326080120848No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT46150.086.227511
GGATGGC35550.085.667797
AGGGATG41500.084.483695
CGCTACG14450.083.2667543
ACGGGTC850.082.941494
TAGGGTA4750.082.126634
TAGCGGG6450.081.612722
ATGGCAC34850.081.5895849
AGGGTAC5500.081.178895
GATGGCA35550.080.3796548
GCTACGA15000.080.2136464
GCGGGAT9450.080.074384
AGGGCAT10050.079.967265
CGAGGGA9200.079.695963
TAAGCGG1950.079.602461
AGGGAAT31150.079.3614965
GGGATGG40800.079.252036
GAGGGTA6350.079.197154
GATGACC21500.079.132028
GAATGCC15750.079.076518
Produced by FastQC (version 0.11.5)