FastQCFastQC Report
Mon 27 Feb 2023
SRR3127989.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127989.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2380631
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT228100.958149330996698No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT39450.16571236785541313No Hit
GCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTG31740.13332599634298636No Hit
CCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTG25180.10577027687197217No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT65750.083.0871051
TAGGGCA11400.075.031474
GAATAGG14700.073.622011
TATAGGG18000.070.5202942
GACCGAT2000.070.49668
ATAGGGA22100.069.752293
CGTAGGG5350.069.4218442
GGTAAGG13150.069.060331
GTACGGG4800.068.56142
AGTAGGG37400.068.5090262
AAGGGTA17650.068.432964
CGAAGGG21900.068.0515442
TAGGGAC12800.067.926414
AGGGTAC9650.067.696215
ACGGGTA1600.067.5592354
ATAGGGC15750.067.438023
GTAGGGA16800.067.4193653
GGGCGAT10500.067.139626
AATAGGG34550.066.812282
AGTTAGG6500.066.5995941
Produced by FastQC (version 0.11.5)