FastQCFastQC Report
Mon 27 Feb 2023
SRR3127988.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127988.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2262643
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT211780.9359850405035173No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT36570.16162514369257544No Hit
GCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTG33820.14947121574194427No Hit
CCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTG26860.11871072900143771No Hit
CTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGC26300.1162357473096728Illumina Single End Adapter 2 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT62550.082.478821
TAGGGCA10500.076.533774
ATAGGGA19150.071.902693
TAGGGTA7950.071.52614
TAGACGG2900.071.353911
AGGGCAT18650.070.8081745
ATAGGGC13300.070.3149643
CGTAGGG5150.070.2635042
GAATAGG12800.069.440841
TAGGGAC10100.069.32844
TAAGGGA20000.069.316773
AAGGGTA16050.068.808034
AGTAGGG35800.068.6538542
AAGAGGG77750.068.3610152
GTAGGGT10250.066.93843
AGAGGGC34400.066.6665043
ATAAGGG31400.066.450712
AGGGTAC10050.066.401545
ATAACGG2350.066.040331
GGTAAGG13650.065.805751
Produced by FastQC (version 0.11.5)