FastQCFastQC Report
Mon 27 Feb 2023
SRR3127987.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127987.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2792742
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT302131.081839998109385No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT45720.16371007418515565No Hit
GCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTG32520.11644469843616059No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27970.10015246664389335No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT85300.081.3467561
TAGGGCA15850.076.507944
ATAGGGC17150.075.915713
ATAGGGA28650.073.496973
ACGGGTA2050.073.368934
CGTAGGG6750.073.1141742
GAATAGG17950.072.071821
GTACGGG6150.071.076152
AGGGCAT23550.070.250965
TATAGGG23400.070.101232
ATAAGGG38350.069.7369462
TAGAGGG50050.069.493422
AATAGGG41550.068.4384842
TAAGGGA25550.068.4333953
AGAGGGC41400.068.1188053
ATGCGGG13150.067.554162
GGTAAGG14700.067.524661
GTAGGGA17450.067.068723
TAGGGAC14050.066.572194
AAGGGTA20950.066.408514
Produced by FastQC (version 0.11.5)