FastQCFastQC Report
Mon 27 Feb 2023
SRR3127985.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127985.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2749424
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT219480.7982762935072947No Hit
GCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTG55690.2025515162448571Illumina PCR Primer Index 7 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTG46480.1690535908612131Illumina PCR Primer Index 7 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC43130.1568692206076618TruSeq Adapter, Index 23 (96% over 25bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC37720.13719237192953868No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT32140.11689721192511596No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT71000.082.672661
ATAGGGC14550.077.517263
CGTAGGG6850.074.78012
AGTAGGG41250.069.723242
TAGGGTA9450.069.621984
TAGGGCG5150.069.351614
TAAGGGA24000.069.1215743
ATAGCGG6950.069.027331
AGGGATG35850.068.8233955
ACGGGAT6950.068.2946554
ATAGGGA25050.068.28793
GAATAGG17300.067.151631
TATAGGG19900.066.8318562
AATAGGG39750.066.679462
AGATAGG15700.066.505721
TAGCGGG14000.066.464122
AGAGGGC35650.065.5159453
AGGGTAA14800.065.4141165
AGGGAAT33350.065.386495
AAGGGTA21200.065.172084
Produced by FastQC (version 0.11.5)