FastQCFastQC Report
Mon 27 Feb 2023
SRR3127984.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127984.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3398298
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT324770.9556842866635005No Hit
GCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTG54210.15952103082189967Illumina PCR Primer Index 7 (95% over 22bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC49100.14448409174239576No Hit
CCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTG46880.13795140979396156Illumina PCR Primer Index 7 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC45390.13356686199974221TruSeq Adapter, Index 23 (96% over 25bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT40590.11944214427339803No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT99000.080.9617161
ATAGGGC18900.074.8522343
CGTAGGG9600.074.4170462
TAGCGGG16700.070.922522
AGTAGGG55450.069.7587052
TAAGGGA31800.069.613563
TAGGGCA17600.068.6310354
ATAGGGA33000.068.079143
TAGGGTA13800.067.775714
AGGGATG41600.067.334915
AGGGCAT24750.067.0327155
TAGAGGG57350.066.546122
GTAAGGG51350.066.4501952
ATAAGGG49400.066.218962
AGAGGGC46850.065.910693
AGATAGG19350.065.876971
ATAGCGG8000.065.852661
AGGGAAT41450.065.084075
GAATAGG20350.064.951211
AAGAGGG113800.063.8509452
Produced by FastQC (version 0.11.5)