FastQCFastQC Report
Mon 27 Feb 2023
SRR3127983.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127983.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1818566
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT735924.046704931248027No Hit
CGCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCT55830.3070001308723467No Hit
CGTTCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTT54280.2984769318243055No Hit
CGTTTCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCT52120.286599441538003No Hit
CCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTG33980.18685051848544404No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT29730.16348045657952473No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTC23650.13004752095882138No Hit
CTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGC21690.11926979829162097No Hit
CGTCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTC20100.11052664571975941No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT203200.090.42391
ATAGGGC12500.078.5799943
AGGGATG27900.074.960345
TAGGGTA4600.072.539794
TAGGGCA7800.070.4964144
GAGGGAT20700.070.1558464
AGAGGGC29300.069.7746053
AGGGAAT20700.067.885445
AGGGTAC6400.067.559065
TAGGGAT11550.067.546354
GTAGGGA11750.067.196583
AAGAGGG59500.067.174532
GAATAGG10450.067.106891
AAGGGTA10450.067.010944
AAGGGCG6600.066.935994
AGTAAGG9850.066.894271
AGTAGGG26750.066.622072
GAGGGCT25800.066.488874
AGTTAGG5100.066.444561
AGGGACT19750.066.3915565