FastQCFastQC Report
Mon 27 Feb 2023
SRR3127981.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127981.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2137525
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT947774.433959836727055No Hit
CGTTCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTT60380.28247622834820646No Hit
CGCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCT59410.2779382697278394No Hit
CGTTTCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCT55740.26076887989614156No Hit
CCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTG40580.1898457327984468No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT34450.16116770564086969No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTC27420.12827920141284896No Hit
CTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGC23760.11115659465971159No Hit
CGTCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTC22810.10671220219646553No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22620.10582332370381632No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT256500.090.569631
GTAGGGA14150.073.077253
AGTAGGG31050.072.9644852
AGGGATG32900.072.855065
ATAGGGC12800.071.2373353
TAGGGTA7250.069.36844
GAGGGAT23900.069.027974
AGGGAAT26400.068.539715
TAGGGCA9850.067.759164
GTTTTTT346100.067.224822
TAGGGAT13650.067.145654
AGAGGGC30250.067.123443
AGGGCAT15550.067.097765
GTAGGGC12100.066.424253
TAGAGGG36350.066.2051242
ATAGGGA19000.065.3079763
AAGGGAT31950.065.023054
ATGAGGG30650.064.868812
GTAAGGG28050.064.849132
AGTTAGG6100.064.770971