FastQCFastQC Report
Mon 27 Feb 2023
SRR3127980.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127980.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2713493
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT232790.8578979197661464No Hit
GCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTG37730.13904587187068476No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC33480.12338340286855355No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT32920.12131964224709627No Hit
CCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTG31620.11652876937585613No Hit
CTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTGC29680.10937931293723624No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT68850.085.04991
ATAGCGG5200.076.038451
TAGGGTA10100.075.847774
CGTAGGG8750.074.697742
GTAGGGA23000.073.7659453
AGGGATG45600.072.5578465
AGGGAAT30650.072.374925
AGTAGGG42900.071.464612
TAAGGGA25900.070.4058153
TAGCGGG13500.069.662162
ATAGGGA25550.069.162953
AAGGGTA20800.068.688944
AGGGTAC13550.068.328755
ATAGGGC16150.067.5137253
AGGGTAT16100.067.431475
AGGGACT39900.067.3751455
AGGGCAT22000.067.292165
GAGGGTA17350.067.178274
TAGGGAT19200.066.824854
AGGGTAG11700.066.680495
Produced by FastQC (version 0.11.5)