FastQCFastQC Report
Mon 27 Feb 2023
SRR3127979.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127979.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2483140
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT208700.8404681169809194No Hit
GCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTG42340.17050991889301448No Hit
CCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTG35870.14445419911885757No Hit
CTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTGC34030.13704422626191032No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC29140.11735141796274072No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT29020.1168681588633746No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT64250.083.233261
AGGGATG41100.074.66895
GTACGGG4900.073.850642
GTAGGGA20350.072.976373
TAGGGTA8150.072.656184
AGGGAAT29500.071.849365
CGTAGGG7200.071.799232
TAAGGGA23150.071.0520553
AAGGGTA20350.070.8979344
ATAGGGA23900.070.0022053
AGGGTAC12700.069.5700765
AGGGTAT14250.068.598855
AGTAGGG39000.068.565762
ACGGGAT8300.068.512044
ATAAGGG35150.067.518942
ATAGGGC14350.067.136953
AATAGGG34700.066.63392
TAGGGAA15350.066.437144
GAATAGG14100.066.380961
AGTAAGG15950.066.348541
Produced by FastQC (version 0.11.5)