FastQCFastQC Report
Mon 27 Feb 2023
SRR3127978.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127978.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3132667
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT304230.9711533335652975No Hit
GCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTG43170.1378059014890507No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC38850.12401573483552514No Hit
CCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTG38230.12203659054728766No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT37730.12044050644387035No Hit
CTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTGC35320.11274738106539892No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT89650.082.693751
CGTAGGG8400.078.332322
AGGGTAC14100.075.332375
TAGGGTA10900.075.026554
AGTAGGG49900.072.61832
AGGGATG53900.072.461035
AGGGAAT37750.070.965975
ATAGGGA33550.070.323983
GTAGGGT13100.070.31973
GTAGGGA27450.069.514593
AAGGGTA25550.069.34944
TAGAGGG52400.069.333082
AGGGTAT19150.068.965175
GAGGGAT45500.068.588134
TAAGGGA29550.068.232633
TAGCGGG14200.067.8512342
TAGGGTC9850.067.755484
AAGAGGG105250.067.7416462
ATAACGG2300.067.492081
ATAGCGG5100.067.331671
Produced by FastQC (version 0.11.5)