FastQCFastQC Report
Mon 27 Feb 2023
SRR3127977.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127977.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1990446
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT202581.0177618483495658No Hit
GCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTG30140.1514233493397962No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC26060.13092543078284968No Hit
CCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTG23360.11736063173781153No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT22350.11228639209503799No Hit
CTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGC21740.10922175231078864TruSeq Adapter, Index 13 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT61050.085.663191
TAGACGG1350.083.684171
CGTAGGG4600.077.685712
AGGGATG37050.077.632085
TAGGGCG3600.074.413264
AGTACGG2450.073.010171
GTAGGGA18250.072.878863
TAGGGCA10950.072.535494
TAAGGGA18200.072.304383
TGTAGGG15650.071.5069052
ATAGGGA19150.071.171683
TAGCGGG10950.070.85252
AAGGGAT40400.069.6824654
GAGGGAT29350.069.495974
TAGAGGG35400.069.335012
AGGGAAT24900.068.703695
AGAGGGA47900.068.387273
GGTAAGG10800.067.993391
TAGGGAT17100.067.885794
AGGGCAT16100.067.723615
Produced by FastQC (version 0.11.5)