FastQCFastQC Report
Mon 27 Feb 2023
SRR3127976.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127976.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1853996
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT186791.0074994768057752No Hit
GCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTG33210.17912660005738953No Hit
CCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTG26410.14244906677252808No Hit
CTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGC24100.12998949296546486TruSeq Adapter, Index 13 (95% over 21bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC23230.12529692620696053No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT19450.10490853270449342No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT51150.082.8259051
ATAGCGG3400.076.0625461
TAGGGCA9950.076.042354
AGGGATG32650.074.127065
GAGGGAT25150.073.061994
CGTAGGG4850.071.7058262
TAGGGCG3450.070.833224
TAAGGGA15800.070.792023
AGTAGGG29150.070.615492
AGGGAAT22950.069.827165
AGGGCAT14150.069.4132845
GAATAGG9900.069.343341
AGGGTAT10650.069.279185
AGTACGG2050.068.810461
AAGGGTA14150.068.749044
TAGCGGG8700.068.603922
GGTAAGG10700.068.553221
ATAAGGG27050.068.453142
AAGGGCA28350.068.296264
ATAGGGC11300.068.2071463
Produced by FastQC (version 0.11.5)