FastQCFastQC Report
Mon 27 Feb 2023
SRR3127975.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127975.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2297695
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT269271.1719135916646901No Hit
GCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTG31860.1386607012680099No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC30020.13065267583382476No Hit
CCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTG26580.11568115002208736No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT24930.10850004025773656No Hit
CTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGC23720.10323389309721265TruSeq Adapter, Index 13 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT71950.084.817681
TAAGGGA23700.075.363073
AGGGATG42500.072.987755
ATAGGGC14000.071.847063
TAGCGGG13700.071.018762
TAGGGCA13700.070.6756744
CGTAGGG7050.070.670812
GGTAAGG15050.070.656011
TAGACGG3150.070.204431
AGGGAAT29550.069.6645055
GAGGGAT34100.069.3325044
AGGGCAT20950.069.321735
TGTAGGG18400.068.460532
ACGGGTA3850.068.367644
GTAAGGG36050.068.189852
ATAGGGA25200.067.892863
AGAGGGC34150.067.7173
GTAGGGA20200.067.711883
TAGAGGG40300.067.6466452
ATAAGGG32350.067.271332
Produced by FastQC (version 0.11.5)