FastQCFastQC Report
Mon 27 Feb 2023
SRR3127974.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127974.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2560952
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT241550.9432039335372159No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT29600.11558201793707966No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT73900.083.050571
ATAGGGC16150.073.0422743
AGTAGGG43850.071.961042
AGATAGG15250.071.293771
CGTAGGG7800.071.142952
GAATAGG15700.069.849891
TAGGGCA14200.068.841144
AAGGGTA22300.068.70464
AGGGTAC12950.068.227775
AGGGATG36300.068.100795
AGTAAGG18050.067.796241
TAAGGGA21900.067.598883
GGTAAGG15750.067.237481
ATGAGGG42750.067.1024252
GTAAGGG41350.066.87232
GACCGAT2250.066.840638
ATAGGGA22850.066.433843
ATGCGGG14300.066.429322
ACGGGAT8100.066.144374
TAGAGGG48600.066.088962
Produced by FastQC (version 0.11.5)