FastQCFastQC Report
Mon 27 Feb 2023
SRR3127973.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127973.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2400317
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220150.9171705237266579No Hit
GCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTTCTG26640.11098534068625103No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT26250.10936055529332167No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT70000.084.513891
ATAGGGC14450.076.757033
AGTAGGG39400.074.313182
ATAGCGG4950.072.2027741
CGTAGGG8150.072.081932
ATAGGGA21650.071.852873
AGATAGG14800.070.2224661
GAATAGG14750.070.141681
TAGGGCA12750.070.032454
ATAGGGT10900.069.8493653
AAGGGTA20000.069.31834
GGTAAGG15500.068.871511
AGGGTAC12700.068.829435
AATAGGG37450.068.51962
AGTAAGG15200.068.37451
AGGGATG35900.068.072835
AAGGGGC68450.068.038714
ATGCGGG12300.068.012522
GATAGGG37350.067.193092
AGGGTAT15550.066.7923665
Produced by FastQC (version 0.11.5)