FastQCFastQC Report
Mon 27 Feb 2023
SRR3127971.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127971.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1238912
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144261.1644087715673106No Hit
GCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTG60240.4862330819299514No Hit
CCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTG55070.4445029186899473No Hit
CTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGC53960.43554344457072014TruSeq Adapter, Index 21 (95% over 22bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC21860.17644513896063643No Hit
AGCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCT20840.16821210868891415No Hit
CGCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCT18240.14722595309432793No Hit
AAAGCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTT17190.13875077487343734No Hit
GGCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCT16910.13649072734786652No Hit
ACTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTG15120.12204256638082447TruSeq Adapter, Index 21 (95% over 21bp)
ACCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCT14770.11921750697386094No Hit
AGAGCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTT14280.11526242380411199No Hit
GGAGCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTT12400.10008781898956504No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT39700.083.857171
TAGGGCG2300.079.6903844
AGGGTAC7400.076.846275
AGGGAAT21850.076.141425
CACGGGT1050.076.090213
AGGGATG17800.075.247835
TAAGGGA12000.074.803393
TAGGGCA4850.074.6136254
GTAGGGT5300.074.485643
GCATAGG3800.074.3496551
ACGGGTA1900.074.205624
AGTAGGG16950.072.965612
AGTAAGG6400.072.8394241
GGGAATG25150.072.504156
AGAGGGC14600.072.104823
ATAGGGA12300.071.832653
GAATAGG7150.071.7846761
CGTAGGG2950.071.7336042
CGAAGGG9050.071.7071842
GTAGGGC6450.071.406133
Produced by FastQC (version 0.11.5)