SRR3127970.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3127970.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1167724
Sequences flagged as poor quality	0
Sequence length	100
%GC	42

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13172	1.1280062754555014	No Hit
GCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTG	6864	0.5878101332164107	No Hit
CTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGC	6141	0.5258948176110109	TruSeq Adapter, Index 21 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTG	5907	0.505855835796815	No Hit
AGCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCT	2247	0.19242560742093165	No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC	2007	0.171872805560218	No Hit
CGCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCT	1911	0.16365168481593254	No Hit
GGCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCT	1882	0.16116822125776298	No Hit
AAAGCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTT	1773	0.15183382374602217	No Hit
ACTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTG	1732	0.14832272009481692	TruSeq Adapter, Index 21 (95% over 21bp)
ACCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCT	1565	0.134021395466737	No Hit
AGAGCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTT	1517	0.12991083509459428	No Hit
TCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTG	1388	0.11886370409446069	No Hit
GAATCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTT	1324	0.1133829569316037	No Hit
GGAGCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTT	1302	0.11149895009437162	No Hit
GAAGCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTT	1301	0.11141331341995198	No Hit
GCCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCT	1224	0.10481928948963967	No Hit
ATAGCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTT	1179	0.10096563914075585	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
TACGGGT	100	89.29011	3
CGTTTTT	3525	85.51579	1
AGGGATG	1610	80.56596	5
AGTACGG	130	79.58418	1
AGGGAAT	2005	77.82016	5
TAGGGCA	545	77.606094	4
TAAGGGA	1110	76.63116	3
ACGGGTA	130	75.91468	4
AGGGTAA	625	75.19489	5
GAATAGG	610	74.00973	1
AGGGTAT	745	73.17624	5
GGGAATG	2345	72.74986	6
GAAGGGC	1595	72.18636	3
GGGCGAT	385	72.02108	6
AGTAGGG	1545	71.78493	2
GGTAAGG	630	71.66021	1
TAGGGAT	855	71.45408	4
TCCGCTA	395	71.43344	1
CGAAGGG	845	71.187386	2
AGGGTCA	595	71.08761	5

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content