FastQCFastQC Report
Mon 27 Feb 2023
SRR3127969.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127969.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1382934
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT198801.4375234103724401No Hit
GCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTG63880.4619164761297358No Hit
CTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGC63080.45613167367350865TruSeq Adapter, Index 21 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTG60800.4396449866732614No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC24950.1804135266035834No Hit
AGCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCT23710.17144708279643134No Hit
AAAGCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTT19150.13847370879593673No Hit
CGCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCT18130.1310980856642472No Hit
ACTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTG17690.12791644431332225TruSeq Adapter, Index 21 (95% over 21bp)
ACCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCT16000.11569604912454245No Hit
GGCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCT15800.11424984851048567No Hit
AGAGCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTT14890.10766963571652732No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14250.10304179375154561No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT14200.10268024359803143No Hit
TCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTG13940.10080018279975762No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT51800.086.028481
ACGGGTG2200.081.179954
GGGCGAT4900.077.694896
AGTAGGG19850.077.4239042
TAGGGCA6200.076.5627444
AGGGTAC6650.075.6250465
TGTAGGG9200.075.606952
AGGGAAT24000.075.005145
GTAGGGT5750.074.380893
AGGGATG19950.074.21155
TAGGGTA6400.074.1701664
AGGGCAT10750.073.8893365
TAAGGGA11200.073.435813
GAATAGG7950.073.396711
CTACGAC5800.072.9319845
AGAGGGT14900.072.548663
GGTAAGG6850.072.1308061
GTAGGGA10050.072.018243
ATAGGGA13750.071.780173
AGACGGG8950.070.892232
Produced by FastQC (version 0.11.5)