FastQCFastQC Report
Mon 27 Feb 2023
SRR3127968.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127968.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2625760
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT239420.9118121991347267No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT32800.12491621473401987No Hit
GCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTG31540.12011760404606667No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT73800.083.337091
ACGGGTA4500.072.06394
TAGGGCA12700.070.312274
AGTAGGG46500.068.35552
ATAGCGG5050.068.022191
AGGGTAC11700.067.8862845
ATAGGGC14950.067.5893863
AGGGAAT35400.066.779935
CGTAGGG8900.066.56722
ATAAGGG38050.066.358932
ATAGGGA24750.066.272213
AGAGGGC38000.065.3027653
GGGCGAT12650.065.017276
GTAGGGA23600.064.921253
TAGAGGG51800.064.9016952
AAGGGCA38550.064.614914
TAGGGAC15800.064.5481644
AGGGATG37000.064.527265
GTAAGGG38400.064.407172
GTACGGG5550.064.387322
Produced by FastQC (version 0.11.5)