FastQCFastQC Report
Mon 27 Feb 2023
SRR3127967.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127967.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2419361
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT218580.903461699184206No Hit
GCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTG34600.14301296912697198No Hit
CCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTG29540.1220983557228541No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT28890.11941169589821445No Hit
CTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGC28060.11598103796828997No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT66750.082.994371
AGTAGGG40100.072.430312
CGTAGGG7550.072.208332
ATAACGG2450.071.021571
ATAGGGC15650.069.670663
AGGGTAC10900.069.417145
ATAGCGG4700.068.0402151
ACGGGTA3250.067.963044
GTAGGGA20000.067.676643
CGAAGGG24450.067.469042
AGAGGGC35450.067.347853
AGGGAAT30950.067.116445
TAGGGCG5050.067.00384
ACGGGAT6650.066.4300464
TAGGGCA11700.066.276024
ATAAGGG33600.065.880652
AATAGGG38600.065.748032
AGTAAGG14400.064.98981
TAACGGG8200.064.765072
TAAGGGA18400.064.621793