FastQCFastQC Report
Mon 27 Feb 2023
SRR3127966.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127966.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3033877
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT313801.0343201125160972No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT39400.12986683375759794No Hit
GCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTG34030.11216670946119438No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT95300.084.85041
ATAGGGC20100.070.850863
TAGGGCA15700.070.649794
CGTAGGG9600.070.500112
AGTAGGG52950.070.477922
AGGGTAC15250.070.266655
GTAGGGA27500.069.38923
TAAGGGA26350.068.136733
TAGGGTA13550.067.638484
CGAAGGG30750.067.404982
AGGGAAT37550.067.212275
ATAAGGG42600.067.190242
TAGAGGG60700.067.13192
GGGCGAT14800.066.6870966
AGAGGGC43150.066.551673
TGTAGGG26200.066.374152
GTAAGGG44650.065.473792
AGTAAGG20650.065.378171
ATAGGGA30800.065.00663
GTAGGGT14750.065.003493
Produced by FastQC (version 0.11.5)