FastQCFastQC Report
Mon 27 Feb 2023
SRR3127964.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127964.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2288406
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT817783.5735791638371865No Hit
CGTTTCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCT80360.3511614634815675No Hit
CGTTCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTT79130.3457865431221558No Hit
CGCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCT70070.30619566632844No Hit
CCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTG45240.19769219273153452No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTC38310.16740910485289762No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT37580.16421911146885648No Hit
CGTCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTC33120.14472956284855046No Hit
CTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC30960.13529067831494937TruSeq Adapter, Index 13 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT233100.089.839011
GAATAGG13300.075.685761
AGGGATG34750.074.6562355
ACGGGTA2600.074.111024
AGTAGGG35650.071.848682
AGGGAAT23150.071.6647345
ATAGGGC16300.071.506563
TAGGGCA8900.070.231834
GAGGGAT25600.069.761524
AGATAGG11950.069.671861
AGAGGGC32500.069.557433
AGGGTAC8800.068.895185
ATAGGGA19650.068.882953
GGATGGC29000.066.930557
AAGGGTA14450.066.674284
AGTAAGG14050.065.9541241
GATAGGG30500.065.951662
CGTTTCT10850.065.897041
ATAGAGG17200.065.63491
GTAGGGT9100.065.590983