FastQCFastQC Report
Mon 27 Feb 2023
SRR3127962.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127962.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2346903
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT581942.4796082326367985No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT36400.15509801640715445No Hit
CGTTCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTT33050.14082388577627622No Hit
CGCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCT30400.12953240930707405No Hit
CGTTTCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCT28500.12143663372538192No Hit
CCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTG26440.11265910862102099No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT174850.088.166821
ATAGGGC13800.073.218613
GAATAGG15000.071.527081
ATAGGGA18700.069.865913
AGTACGG2900.069.77461
AGTAGGG37750.069.502672
AGAGGGC33900.069.454463
AGATAGG11500.068.744561
TAGAGGG41200.068.704232
AGGGATG32150.068.557355
TAAGGGA19700.067.989333
TAGGGCA13100.067.803584
GTACGGG5400.067.047292
AAGAGGG78800.066.890372
ATAGCGG5150.064.87511
AGGGCAT17450.064.636525
AGGGACT31150.064.572545
AATAGGG35650.064.496122
TAGCGGG13500.064.435052
ATAGAGG15950.064.316541
Produced by FastQC (version 0.11.5)