FastQCFastQC Report
Mon 27 Feb 2023
SRR3127961.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127961.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2179206
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT531842.440521914862569No Hit
CGTTCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTT35800.16428001758438623No Hit
CGCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCT33320.15289972586345668No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT32850.15074297702924827No Hit
CGTTTCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCT30590.14037222731582052No Hit
CCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTG29220.13408553390546832No Hit
CTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC23790.10916820162940082No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT152950.088.5053561
ATAGGGC12650.073.557013
TAGGGCA11600.072.112654
AGTAGGG34300.071.5197142
GAATAGG12650.070.64661
GTAGGGT8750.068.209533
GATAGGG30400.067.8641362
CGTAGGG7800.067.479652
ATAGCGG5250.067.1939541
AGAGGGC31150.067.135373
ATGAGGG33600.066.995512
TAGGGTA7650.066.959854
AGGGATG28500.066.946925
ATAGGGT9200.066.916453
ATAGGGA17500.066.329743
AGGGACT27100.065.549885
GTACGGG5600.065.456982
TAGAGGG36750.065.217222
AGATAGG13000.065.126451
AAGGGTA13900.064.913634
Produced by FastQC (version 0.11.5)