FastQCFastQC Report
Mon 27 Feb 2023
SRR3127960.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127960.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2720154
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT744872.738337608826559No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT42440.1560205782466728No Hit
CGTTCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTT34450.12664724129589722No Hit
CGCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCT31030.11407442372748014No Hit
CGTTTCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCT30460.11197895413274396No Hit
CCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTG30190.11098636327207946No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT212000.088.479381
ATAGGGC16650.073.112383
AGGGATG36400.071.2733465
AGTAGGG41800.070.9512
ACGGGTA4350.070.229814
GTAGGGT11000.070.0739443
TAGGGCA14450.069.9307254
GTACGGG6400.069.7668462
TAGAGGG49050.069.087852
TAGGGTC8550.067.0642554
AAGAGGG94250.066.823442
AGAGGGC38900.066.816083
CGTAGGG7600.066.7906342
TAGGGTA10000.066.2699364
ATGAGGG43800.066.2089162
GAGGGAT33800.066.1892854
GAATAGG18250.065.7533651
AGATAGG14600.065.753361
ATAGAGG20700.065.7004241
ATAGGGA23050.065.658413
Produced by FastQC (version 0.11.5)