FastQCFastQC Report
Mon 27 Feb 2023
SRR3127958.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127958.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences478120
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37490.7841127750355559No Hit
GCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTG25350.5302016230235087No Hit
CTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC24410.5105412867062662No Hit
CCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTG23150.4841880699406007No Hit
AGCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCT9010.18844641512590982No Hit
AGAGCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTT8110.16962268886472015No Hit
ACTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTG6970.14577930226721325No Hit
CGCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCT6700.14013218438885636No Hit
GAATCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTT6680.1397138793608299No Hit
TTAATGGGCGAGTCCTCCCTCCCAATTGGAAATATGAATCATCTACAGCC6390.1336484564544466No Hit
GATCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTC6350.13281184639839372No Hit
GGCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCT6210.12988371120220865No Hit
AAAGCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTT5980.12507320337990463No Hit
ACCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCT5670.11858947544549486No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA5130.10729523968878106No Hit
AGGCCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTT5040.10541286706266209No Hit
AATGCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTT4950.10353049443654312No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAGGG2900.085.890432
ACACGGG1000.084.593972
CACGGGT500.084.593973
ACGGGTA500.084.593974
CGTTTTT14050.084.3458561
TAGCGGG1800.083.549612
CGTAGGG1350.083.54962
ATAGCGG1150.081.784631
ATAGGGT1500.081.460863
GGGTATC3000.081.460866
AGGGTCA1850.081.291515
ATTGCGG353.8449798E-880.616271
AGGGCGT353.8617145E-880.565695
AGGGAAT10500.079.670525
GGGCATA650.079.53286
AGGGTAT2500.078.954385
CGGGATA600.078.327765
GGGCGAT1750.077.880176
GGAATCG551.8189894E-1276.903627
CGAGGGA1550.075.8010563
Produced by FastQC (version 0.11.5)