FastQCFastQC Report
Mon 27 Feb 2023
SRR3127957.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127957.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences560899
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54320.9684452994211079No Hit
CTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC23940.4268148097964161No Hit
GCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTG23750.4234273906710477No Hit
CCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTG23110.41201713677507No Hit
AGCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCT8030.1431630293510953No Hit
TTAATGGGCGAGTCCTCCCTCCCAATTGGAAATATGAATCATCTACAGCC7700.1372796171859818No Hit
AGAGCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTT6890.12283851459888499No Hit
GAATCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTT6650.11855966938789336No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA6460.11517225026252498No Hit
CGCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCT6420.11445910939402637No Hit
ACTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTG6370.11356768330840313No Hit
ACCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCT6290.1121414015714059No Hit
GATCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTC6290.1121414015714059No Hit
AAAGCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTT5890.10500999288641984No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATAT201.8366658E-594.004198
ACGGGTA353.583409E-1093.9958044
CGTTTTT20000.086.318111
ATAGCGG950.084.176561
AGGGTAT3150.083.559285
CGTAGGG1350.083.551832
TAAGCGG401.027729E-982.319721
CGAAGGG3650.081.119672
TAGCGGG2100.080.567832
ATGGGCG2000.079.896444
TACGAGG1300.079.605891
AGGGCGA2600.079.542015
ATAGGGC2750.078.614683
AAGGGTA3950.078.5281454
AGACGGG2950.078.064322
TGAGGGC4050.076.589183
GGGGAGC34800.076.5817
GTAGGGC2150.076.508223
GGAGCCA21650.076.4191069
ACGGGCC800.076.37164
Produced by FastQC (version 0.11.5)