FastQCFastQC Report
Mon 27 Feb 2023
SRR3127954.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127954.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2336780
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1059244.532904252860774No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCT70180.3003278015046346No Hit
CGTTCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTT67280.2879175617730381No Hit
CGCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCT60080.2571059320945917No Hit
CCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTG38780.16595486096252107No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTC33490.14331687193488474No Hit
CGTCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTC28680.12273299155247819No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT27470.11755492600929483No Hit
CTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGC26210.11216289081556671No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24240.10373248658410292No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT279150.090.2839051
ATAGGGC14900.075.386173
GTAGGGA16750.072.391073
AGGGATG32300.072.3190165
TAGGGTC6700.072.250774
AGTAGGG37100.071.573882
TAGGGTA8550.071.459064
GAATAGG14950.071.4351
ATAGCGG4600.069.5468441
GTAGGGT10200.068.654053
TAGAGGG38200.067.9134452
ATAGAGG15600.067.855381
AGAGGGC30200.067.8515243
TAGGGAT13050.067.706044
TAGGGCA10050.067.340524
ATAGGGA18350.067.1035163
AAGGGTA15550.067.096934
GAGGGAT23800.066.9426354
GTACGGG4150.066.816542
GTTTTTT379150.066.7505342