FastQCFastQC Report
Mon 27 Feb 2023
SRR3127953.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127953.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences817854
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82241.005558449307578No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC41130.5029014958660103No Hit
GCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTG26060.31863877904858323No Hit
CCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTG20740.25359049414687707No Hit
CTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGC20010.24466469565472565TruSeq Adapter, Index 22 (95% over 21bp)
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC13300.16262071225426544No Hit
AAAGCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTT9740.11909216070349965No Hit
AGCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCT9260.11322314251687954No Hit
TCCGCTACGACCAACTCATACACCTGTCTCTTATACACATCTGACGCGTC8240.10075147887031181No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT27050.086.809691
ATAGCGG1450.084.380141
AGGGATG29100.083.82075
GCTACGA7400.081.928484
GGATGGC23300.081.489647
TAGGGCG1850.081.293384
AGAGGGC12100.081.177913
GTACGGG1800.080.96532
CGAGGGA6300.080.567553
GAGGGAT16000.079.0149464
GATGGCA22200.078.959848
CGCTACG7750.078.8349153
CGAAGGG6550.078.233892
AAGGGCA11300.078.1909264
TCCGCTA7850.077.93071
TACGAGG1700.077.5075451
GGGCGAT1950.077.12456
TAGGGAT12000.076.762974
AGGGCGT1900.076.680525
TAGGGCA5650.076.527294