Basic Statistics
Measure | Value |
---|---|
Filename | SRR3127953.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 817854 |
Sequences flagged as poor quality | 0 |
Sequence length | 100 |
%GC | 43 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 8224 | 1.005558449307578 | No Hit |
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC | 4113 | 0.5029014958660103 | No Hit |
GCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTG | 2606 | 0.31863877904858323 | No Hit |
CCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTG | 2074 | 0.25359049414687707 | No Hit |
CTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGC | 2001 | 0.24466469565472565 | TruSeq Adapter, Index 22 (95% over 21bp) |
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC | 1330 | 0.16262071225426544 | No Hit |
AAAGCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTT | 974 | 0.11909216070349965 | No Hit |
AGCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCT | 926 | 0.11322314251687954 | No Hit |
TCCGCTACGACCAACTCATACACCTGTCTCTTATACACATCTGACGCGTC | 824 | 0.10075147887031181 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGTTTTT | 2705 | 0.0 | 86.80969 | 1 |
ATAGCGG | 145 | 0.0 | 84.38014 | 1 |
AGGGATG | 2910 | 0.0 | 83.8207 | 5 |
GCTACGA | 740 | 0.0 | 81.92848 | 4 |
GGATGGC | 2330 | 0.0 | 81.48964 | 7 |
TAGGGCG | 185 | 0.0 | 81.29338 | 4 |
AGAGGGC | 1210 | 0.0 | 81.17791 | 3 |
GTACGGG | 180 | 0.0 | 80.9653 | 2 |
CGAGGGA | 630 | 0.0 | 80.56755 | 3 |
GAGGGAT | 1600 | 0.0 | 79.014946 | 4 |
GATGGCA | 2220 | 0.0 | 78.95984 | 8 |
CGCTACG | 775 | 0.0 | 78.834915 | 3 |
CGAAGGG | 655 | 0.0 | 78.23389 | 2 |
AAGGGCA | 1130 | 0.0 | 78.190926 | 4 |
TCCGCTA | 785 | 0.0 | 77.9307 | 1 |
TACGAGG | 170 | 0.0 | 77.507545 | 1 |
GGGCGAT | 195 | 0.0 | 77.1245 | 6 |
TAGGGAT | 1200 | 0.0 | 76.76297 | 4 |
AGGGCGT | 190 | 0.0 | 76.68052 | 5 |
TAGGGCA | 565 | 0.0 | 76.52729 | 4 |