FastQCFastQC Report
Mon 27 Feb 2023
SRR3127952.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127952.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences761279
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76231.0013411640147698No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC37840.49705824014585975No Hit
GCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTG27120.356242586489316No Hit
CCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTG23280.30580115831383764No Hit
CTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGC22160.2910890750959898TruSeq Adapter, Index 22 (95% over 21bp)
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC12660.16629908351603026No Hit
AGCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCT10760.14134108520003835No Hit
AAAGCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTT10440.13713763285208183No Hit
TAGACCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCT8910.11703987631341466No Hit
TCCGCTACGACCAACTCATACACCTGTCTCTTATACACATCTGACGCGTC8720.11454407648181547No Hit
CGCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCT8550.11231099242196357No Hit
AGAGCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTT7740.1016710036661986No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTATG156.903974E-493.9908758
CGTTTTT20800.089.061151
GGCCGAT700.087.277247
ACGGGTA1150.085.817764
AGGGATG23900.085.535625
ATAGCGG1700.082.971271
AGAGGGC11050.082.507823
AAGGGCA10200.082.472384
GGATGGC21400.080.3753747
GCTACGA6800.080.168684
AGGGCAA6650.079.85695
GCGGGAT4450.079.205784
TCCGCTA6950.079.151011
CGCTACG6900.079.006813
AGGGAAT15300.078.018575
GAGGGAT15300.077.404254
CTACGAC7050.077.325825
ACGACCA7250.076.489127
AGTACGG800.076.402711
CGAGGGA5850.076.3173753
Produced by FastQC (version 0.11.5)