FastQCFastQC Report
Mon 27 Feb 2023
SRR3127951.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127951.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences947562
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110621.1674170133458286No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC48820.51521694622621No Hit
GCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTG27380.28895206857176625No Hit
CCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTG24020.25349264744681616No Hit
CTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGC21630.22827002349186648TruSeq Adapter, Index 22 (95% over 21bp)
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC16600.17518642579588461No Hit
AAAGCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTT10400.10975535110103612No Hit
AGCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCT9480.10004622388825217No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT32450.086.6879961
AGGGATG31400.085.325
GGCCGAT1000.084.6015247
CGCTACG8250.084.316673
ACGACCA8250.083.177257
GCTACGA8550.081.3581854
GAGGGAT20000.081.3114554
TCCGCTA8800.081.251881
AGTACGG1100.081.251881
ACGGGTA1100.081.183274
GGATGGC27500.081.012377
CGTAGGG1650.079.759012
ATAGCGG1950.079.60711
GTACGGG1950.079.539892
AGAGGGC13500.079.031053
AGGGCAC8800.078.512775
AGGGAAT19500.078.093715
AAGGGCA12150.077.7544864
ATGGCAC26700.076.9264769
CGAGGGA7400.076.852733
Produced by FastQC (version 0.11.5)