FastQCFastQC Report
Mon 27 Feb 2023
SRR3127949.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127949.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2134916
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT227181.0641168083428107No Hit
GCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTG30040.14070811216928442TruSeq Adapter, Index 23 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTG27220.12749916155951804TruSeq Adapter, Index 23 (95% over 22bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC26390.12361142077721092No Hit
CTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGC26110.1222998937663121TruSeq Adapter, Index 22 (95% over 21bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT24050.11265080218612816No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT69450.083.823291
TAGGGCA11300.075.2769554
ATAGGGC13250.073.065653
AGGGATG31850.071.121285
AGTAGGG36300.069.523172
ATAGGGA18700.069.363243
AGATAGG13900.069.351421
GAATAGG13300.069.2980041
AGGGTAA10650.069.280655
AGGGAAT22200.068.588925
TAGGGTA8350.068.102154
AGGGCAT20150.066.937395
ACGGGAT5450.066.398184
ATAGAGG14350.066.1935651
GGGCGAT8900.066.0057756
CGTAGGG5200.065.975312
AATAGGG32950.065.8943942
AAGGGTA14550.065.891464
TAGCGGG11650.065.754222
TAGGGTC7150.065.728834
Produced by FastQC (version 0.11.5)