FastQCFastQC Report
Mon 27 Feb 2023
SRR3127948.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127948.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2673457
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT324911.2153178450223812No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC34780.1300937325717227No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT30210.11299976023553025No Hit
GCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTG29730.11120433206892799TruSeq Adapter, Index 23 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTG29290.10955852291620924TruSeq Adapter, Index 23 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGC27560.10308750056574689TruSeq Adapter, Index 22 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT93200.085.9629061
TAGGGCA13450.073.034834
ATAGGGC15800.072.881113
ACGGGTA3100.071.259394
AAGGGTA19100.070.624354
GAATAGG16300.070.4230041
TAGGGTA12950.070.0476464
AGTAGGG44300.069.5994952
TAGGGTC7400.069.2310264
AGGGAAT31000.069.1329045
ATAGGGA29100.068.966933
CGTAGGG7850.068.854792
ATAGAGG20100.068.109741
AATAGGG42100.068.1010362
AGGGATG39850.067.4604645
GTAGGGT14150.066.76453
AGGGTAA15650.066.668435
ACGGGAT7050.066.6679154
TAAGGGT13350.066.540573
AGGGTAC12600.066.394345
Produced by FastQC (version 0.11.5)