FastQCFastQC Report
Mon 27 Feb 2023
SRR3127947.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127947.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1943461
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT647053.329369614311787No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCT41920.21569766514481126No Hit
CGTTCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTT41000.2109638423410606No Hit
CGCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCT35630.18333272445395096No Hit
CCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTG25440.13090049144284346No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT21700.11165647265368331No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTC20800.10702555904131855No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT190550.090.05531
ATAGGGC10950.076.3959053
AGGGATG28200.069.661285
AGTAGGG31800.068.75472
TAGGGCA9300.068.220534
GAATAGG12350.068.216221
ATAGGGA18700.067.8557053
TAGGGAT13650.067.826244
AGATAGG10650.067.615051
TAAGGGA16800.065.738953
GATAGGG28050.065.709752
GTAGGGT6750.065.4467853
GTAGGGA13300.065.370883
AGAGGGC26800.065.233763
ATAGCGG4050.065.078051
TATAGGG14500.064.854262
GTACGGG5050.064.2442552
GAGGGAT22350.064.133734
AAGGGAT29800.064.0285954
TGTAGGG14900.063.744352
Produced by FastQC (version 0.11.5)